Phosphorylation of F-actin-Associating G Protein γ12 Subunit Enhances Fibroblast Motility
نویسندگان
چکیده
منابع مشابه
Association of the γ12 subunit of G proteins with actin filaments
Recent studies have suggested an association between heterotrimeric G proteins, which play a major role in transmembrane signal transduction, and intracellular components. We therefore examined the subcellular localization of isoforms of G protein γ subunits in Swiss 3T3 and C6 glioma cells, mainly containing the γ5 and γ12 subunits. Immunocytochemical double staining with phalloidin showed co-...
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To assess the role of myosin II heavy chain (MHC) phosphorylation in basic motility and natural chemotaxis, the Dictyostelium mhcA null mutant mhcA(-), mhcA(-) cells rescued with a myosin II gene that mimics the constitutively unphosphorylated state (3XALA) and mhcA(-) cells rescued with a myosin II gene that mimics the constitutively phosphorylated state (3XASP), were analyzed in buffer and in...
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Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1999
ISSN: 0021-9258
DOI: 10.1074/jbc.274.17.12124